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2×Taq PCR MasterMix含染料

2×Taq PCR MasterMix含染料

產(chǎn)品編號:RTC3104-02

產(chǎn)品規(guī)格:500μl

相關(guān)規(guī)格:
數(shù)量
價格 ¥60


2×Taq PCR MasterMix

 產(chǎn)品包裝:

 

 

目錄號

包裝

價格

2×MasterMix(不含染料)

RTC3103-02

500μl

60

RTC3103-03

5×500μl

240

2×MasterMix(含染料)

RTC3104-02

500μl

60

RTC3104-03

5×500μl

240

注:以50μl PCR反應(yīng)體系為例,每次使用25μl 2×MasterMix,500μl可做20 50μl PCR反應(yīng)。

 儲存條件: -20℃可長期保存;4℃穩(wěn)定貯存3個月。經(jīng)常使用時,一旦融化后請4℃貯存,盡量避免反復(fù)凍融。

 產(chǎn)品簡介:

本產(chǎn)品包含Taq DNA聚合酶、dNTPs、MgCl2、反應(yīng)緩沖液、PCR反應(yīng)的增強劑和優(yōu)化劑以及穩(wěn)定劑,濃度為2×。使用時,只需補加實驗所需的引物和模板后即可進行PCR反應(yīng),可最大限度的減少人為誤差,具有快速簡便、穩(wěn)定性好等優(yōu)點。適用于常規(guī)PCR反應(yīng)、復(fù)雜模板(如GC含量高,有二級結(jié)構(gòu))的擴增以及大規(guī)模基因檢測。使用該產(chǎn)品得到的PCR擴增產(chǎn)物末端含有一個A堿基,可以直接用于TA克隆。

 質(zhì)量控制:

經(jīng)檢測無外源核酸酶活性;PCR方法檢測無宿主殘余基因組DNA;能有效地擴增人基因組中的單拷貝基因。

●  使用說明:

1. 冰浴中徹底融化2×MasterMix,混勻后Minispin將溶液收集到管底。

2. 按照下表在0.2ml PCR管中制備反應(yīng)體系:

 

50μl反應(yīng)體系

終濃度

2×MasterMix

25μl

上游引物 10μM

1-5μl

0.2-0.8 μM

下游引物 10μM

1-5μl

0.2-0.8 μM

模板

× μl

10pg-1μg

補至50μl

 

3. 快甩離心將反應(yīng)液收集到管底。

4. PCR儀如果沒有熱蓋加熱的話,補加25μl礦物油。

5. PCR儀上執(zhí)行以下程序:

步驟

溫度

時間

循環(huán)數(shù)

初始變性

94

5 min

1

變性

94

30 sec

25-40*

退火

Tm-5*

30 sec

延伸

72

1 min/kb

最后延伸

72

5 min

1


*
注: PCR 反應(yīng)條件視模板、引物等的結(jié)構(gòu)條件不同而各異。在實際操作中需根據(jù)模板、目的片段的大小、堿基序列和引物的長短等具體情況,設(shè)定最佳的反應(yīng)條件(溫度、時間等)。

產(chǎn)品示例:


2×Taq PCR MasterMix擴增效果比較

M III:RTM403 DNA Marker III

lane 1:Rice 5000 bp

lane 2:Ricie 3000 bp

lane 3: Rice 600 bp


引用RTC3104 2×Taq PCR MasterMix含染料發(fā)表文章

1. [2013 IF=1.63] Preventive effect of total glycosides from Ligustri Lucidi Fructus against nonalcoholic fatty liver in mice

Author: Nianyun Yang, Yiwen Zhang and Jianming Guo

ProductRTC3104 2×Taq PCR MasterMix含染料

Journal: Z. Naturforsch. 2015; 70(9-10)c: 237–241

Institution:Nanjing University of Chinese Medicine

Paper link https://pubmed.ncbi.nlm.nih.gov/26501160/

 

2. [2015 IF=1.377] Expression  and  localization  of  guanine  nucleotide-binding protein  alpha  S  in  the  testis  and  epididymis  of  rams  at different  developmental  stages

Author: Zhen  Li,  Jieli  Lu,  Jia  Chen,  Quanhai  Pang,  Ruipeng  Nan,  Zhiwei  Zhu

ProductRTC3104 2×Taq PCR MasterMix含染料

Journal: Animal  Reproduction  Science. Volume 178, March 2017, Pages 31-39

Institution:Shanxi Agricultural University

Paper linkhttps://www.sciencedirect.com/science/article/pii/S0378432016307187

 

3. [2018 IF=2.9] Integration of gene expression profile data to screen and verify immune-related genes of chicken erythrocytes involved in disease virus Marek’s

Author: Ali Raza Jahejo, Sayyad Ali Raza Bukhari, Fa-Jie Jia, Sayed Haidar Abbas Raza, Mujahid Ali Shah, Nasir Rajput, Anam Ahsan, Sheng Niu, Guan-Bao Ning, Ding Zhang, Yu-Hai Bi, Qi-Hui Wang, Ling-Xia Han, Wen-Xia Tian

ProductRTC3104 2×Taq PCR MasterMix含染料

Journal: Microbial Pathogenesis. 148 (2020) 104454

Institution:Shanxi Agricultural University

Paper linkhttps://doi.org/10.1016/j.micpath.2020.104454

 

4. [2018 IF=1.69] Study on the correlation between BMPR1Bprotein in sheep blood and reproductive performance

Author: Xiaoyan Zhang, Liping Zhang, Weibo Sun, Xia Lang, Jianping Wu, Caiye Zhu,Jianlei Jia, Jinpen Jin, Younfu La, David P. Casper

ProductRTC3104 2×Taq PCR MasterMix含染料

Journal: Journal of Animal Science. 2020 May 1;98(5)

Institution:Gansu Agricultural University

Paper linkhttps://pubmed.ncbi.nlm.nih.gov/32300800/

 

5. [2019 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Author: Dongmei Jiang, DizheWei, Hongtao Li,LiuqingWang,Nan Jiang,Yunchao Li, Meng Wang

ProductRTC3104 2×Taq PCR MasterMix含染料

Journal: Journal of the Science of Food and Agriculture. Volume101, Issue7,May 2021

Institution:Beijing Academy of Agriculture and Forestry Sciences

Paper linkhttps://doi.org/10.1002/jsfa.10901

 

6. [2022 IF=6.9] Galactinol synthase 2 infuences the metabolism of chlorophyll, carotenoids, and ethylene in tomato fruits.

Author: Huidong Zhang, Kunpeng Zhang, Xueya Zhao, Mengxi Bi, Yudong Liu, Shuo Wang, Yi He,Kui Ma, Mingfang Qi

ProductRTC3104 2×Taq PCR MasterMix含染料

Journal: Journal of Experimental Botany Published: 15 March 2024

Institution:College of Horticulture, Shenyang Agricultural University

Paper linkhttps://doi.org/10.1093/jxb/erae121

 

 


 
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