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首頁(yè) > 蛋白生物學(xué) > 蛋白Marker > 變性蛋白Marker

6.5 kD單條蛋白Marker

6.5 kD單條蛋白Marker

產(chǎn)品編號(hào):RA1030M

產(chǎn)品規(guī)格:500次

數(shù)量
價(jià)格 ¥300


6.5 kD單條蛋白Marker

 

貨號(hào)

名稱(chēng)

規(guī)格

RA1030M

6.5 kD單條蛋白Marker

500

產(chǎn)品編號(hào)及規(guī)格:

組份

名稱(chēng)

規(guī)格

貯存

RA1030M-01

6.5 kD單條蛋白Marker

1 mg

-20

TP050-01

2×Tricine多肽上樣緩沖液(變性,還原)

1 ml

-20℃

TP070-01

2×Tricine多肽上樣緩沖液(非變性,非還原)

1 ml

-20℃

儲(chǔ)存、效期及運(yùn)輸:

  -20保存;有效期一年;濕冰運(yùn)輸。

產(chǎn)品簡(jiǎn)介:

   本產(chǎn)品包含一種已知分子量的標(biāo)準(zhǔn)蛋白質(zhì),分子量大小為6.5 kD,可以用來(lái)判斷多肽電泳時(shí)蛋白的分子量(變性還原電泳)。產(chǎn)品配套非變性電泳上樣緩沖液,可用于粗略判斷多肽非變性電泳時(shí)條帶的大小(非變性非還原電泳)。

6.5 kD單條蛋白Marker 以干粉狀態(tài)提供。以每次使用配制好的溶液10 μl(0.2 μg/μl)計(jì)算,該產(chǎn)品可以大約使用500次。

使用說(shuō)明:

. 配制10 mg/ml 6.5 kD蛋白Marker母液:

1 mg 粉末溶于100 μl超純水中,徹底溶解。此溶液-20貯存,有效期一年。

. 按照下表配制Marker溶液

 

變性還原電泳

非變性非還原電泳

組份

配制量:100 μl 濃度0.2 μg/μl

6.5 kD母液 (10 mg/ml)

2 μl

2 μl

滅菌水

補(bǔ)至100 μl

48 μl

2×Tricine多肽上樣緩沖液(變性,還原)

50 μl

-

2×Tricine多肽上樣緩沖液(非變性,非還原)

-

50 μl

1 M DTT

5 μl(可選)

-

 

95處理5分鐘

不要加熱

 

注:由于2×Tricine多肽上樣緩沖液(變性,還原)中DTT含量會(huì)隨貯存時(shí)間延長(zhǎng)而降低,變性還原電泳可以選擇補(bǔ)加DTT。

2.1  取配制好的溶液徹底混勻后,立即上樣電泳,每次上樣10 μl。

2.2  電泳結(jié)束后,染色,觀察結(jié)果。

注:6.5 kD蛋白電泳要使用Tricine系統(tǒng)電泳(貨號(hào):RTD6120和RTD6121),如使用經(jīng)典Laemmli系統(tǒng)會(huì)導(dǎo)致片段不可見(jiàn)。

. 實(shí)驗(yàn)示例:

3.1 6.5 kD單條蛋白變性還原電泳:





使用蛋白Marker產(chǎn)品發(fā)表部分文章列表:

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control 50 (2015) 896e900

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper linkhttp://dx.doi.org/10.1016/j.foodcont.2014.10.040

 

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control  Volume 81, November 2017, Pages 211-217

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper linkhttps://doi.org/10.1016/j.foodcont.2017.05.030

 

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper linkhttps://doi.org/10.1093/jxb/ery351

 

4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

Marker: RTD6127,超低分子量蛋白Marker IV(3.3-45 kD)

Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

InstitutionCollege of Biological Science and Biotechnology, Beijing Forestry University

Paper linkhttps://link.springer.com/article/10.1007/s00217-017-2958-3

 

5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper linkhttps://doi.org/10.1093/jxb/ery351

 

6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110,彩虹預(yù)染超低分子量蛋白(1.2-45 kD)

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

InstitutionBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper linkhttps://doi.org/10.1093/plcell/koab046

 

7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

Marker: RTD6110,彩虹預(yù)染超低分子量蛋白(1.2-45 kD)

Journal: Analytical Methods. 2021,13, 2804-2811

InstitutionDepartment of Chemistry, Fudan University

Paper linkhttps://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

 

8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

Marker: RTD6142 高分子量非變性電泳蛋白質(zhì)Marker II(45-669 kD)

Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

InstitutionCollege of Veterinary Medicine, Hunan Agricultural University,

Paper linkhttps://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

 

9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

Marker: RTD6109 高分子量蛋白質(zhì)Marker(43-200 kD)

Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

InstitutionCollege of Food Science and Technology, Hebei Normal University of Science and Technology

Paper linkhttps://link.springer.com/article/10.1134/S0003683810060116

 

10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

Author: Dong Liu

Marker: RTD6103 低分子量蛋白質(zhì)Marker II(14.4-116 kD)

Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

InstitutionDepartment of Food Processing Technology, Anqing Vocational & Technical College

Paper linkhttps://doi.org/10.1111/1471-0307.12395

 

11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

Marker: Protein Marker

Journal: Biomedical Materials, 2022 Jan 24;17(2)

Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

Paper linkhttps://pubmed.ncbi.nlm.nih.gov/34996052/

 


 
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